We have undertaken the Neuroscience Monoclonal Antibody Sequencing Initiative (NeuroMabSeq), a concerted effort to determine and make publicly available the sequences of monoclonal antibodies valuable to neuroscience research.

We previously created a valuable collection of mouse monoclonal antibodies (mAbs) validated for neuroscience research. As part of a BRAIN Initiative project (NIH U24 NS109113) to enhance dissemination and utility of this valuable resource, we are applying high-throughput DNA sequencing technologies to determine the sequence of the immunoglobulin heavy and light chain variable domains from our collection of hybridoma cells that are the source of these mAbs.

These efforts will allow for permanent archiving of this valuable collection as DNA sequence. The availability of these sequences will also allow researchers to generate plasmids for expression of molecularly defined mAbs, leading to enhanced research transparency and reproducibility. This will also allow for engineering of these antibodies into alternate forms including single chain variable fragments or ScFvs.

This website serves as the public DNA sequence repository for the sequences of these mouse mAb heavy and light chain variable domains and serves as an open resource for enhancing dissemination of this valuable collection of validated mAbs for research purposes.

More information on the project can be found in this publication.. Please cite the paper if you use the data in this database and reference the full mAb ID in your usage.

Disclaimer: The posted sequences are provided “as-is” and without representation or warranty whatsoever, and are for research use only. These sequences are owned by The Regents of the University of California and may only be used for non-commercial research purposes. Any commercial use is prohibited without receipt of prior written permission from The Regents.