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FAQ List

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Why do some entries have more than one light and/or heavy chain sequence? We are displaying any returned sequences that meet the threshold for comprising at least 30% of all Amplicon Sequence Variants (ASVs) from that sample. It is now established that monoclonal hybridomas can express more than one light and/or heavy chain (e.g., Bradbury et al., MABS 10: 539, 2018). Moreover, a subset of these samples are not monoclonal but are oligoclonal, as indicated in the category title. Note that the aberrant light chain sequence derived from SP2/0 myeloma cell partner is not shown.

Definition List

Term Definition
NeuroMab mAbs sequences obtained from the specific subcloned hybridoma cell lines producing the mAbs that comprise the NeuroMab collection (http://neuromab.ucdavis.edu/neuromabs.cfm)
Non-NeuroMab mAbs sequences obtained from subcloned hybridoma cell lines producing other mAbs that passed multiple validation criteria but that are not part of the NeuroMab collection
NeuroMab Alternative Subclones sequences obtained from alternate subcloned hybridoma cell lines producing the mAbs that comprise the NeuroMab collection
Lead oligoclonal Abs sequences obtained from non-subcloned/oligoclonal hybridoma cell lines producing one or more mAbs that passed multiple validation criteria
Other oligoclonal Abs sequences obtained from non-subcloned/oligoclonal hybridoma cell lines producing one or more mAbs that passed one or more validation criteria
Recombinant mAbs sequences obtained from plasmids expressing validated recombinant mAbs (see Andrews et al., 2019: eLife 8:e43322. PMID: 300667360)
NeuroMab mAbs commercially sequenced: sequences independently obtained by outside vendors from the specific subcloned hybridoma cell lines producing the mAbs that comprise the NeuroMab collection