Sequence: 1 -- Star rating: ☆2.68 |
AGCATCACACTGAAAACACACAGACATGAGTGTGCCCACTCAGGTCCTGGGGTTGCTGCTGCTGTGGCTTACAGGTGCCAGATGTGACATCCAGATGACTCAGTCTCCAGCCTCCCTATCTGCATCTGTGGGAGAGACTGTCACCATCACATGTCGAGCAAGTGAGAATATTTACAGTTATTCAGCATGTTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTCTATAATGCAAAAACCTTACCAGAAGGTGTGCCATCAAGGTTCAGTGGCAGTGGATCAGGCACACAGTTTTCTCTGAAGATCAACAGCCTGCAGCCTGAAGATTTTGGGAGTTATTACTGTCAACATCAGTATGGTACTCCGTGGACGTTCGGTGGAGGCACCAAGCTGGAAATCAAACGGGCT |
Sequence 1 corresponding to IMGT amino acids 1-127: |
GACATCCAGATGACTCAGTCTCCAGCCTCCCTATCTGCATCTGTGGGAGAGACTGTCACCATCACATGTCGAGCAAGTGAGAATATTTACAGTTATTCAGCATGTTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTCTATAATGCAAAAACCTTACCAGAAGGTGTGCCATCAAGGTTCAGTGGCAGTGGATCAGGCACACAGTTTTCTCTGAAGATCAACAGCCTGCAGCCTGAAGATTTTGGGAGTTATTACTGTCAACATCAGTATGGTACTCCGTGGACGTTCGGTGGAGGCACCAAGCTGGAAATCAAA |
Sequencing Info
☆ Scoring Info
Amino Acid Information
Blat Sequence
Percent Support |
ASV Count |
Platform |
Plate |
Total Reads |
SMARTindex |
75.80% |
1803 |
Illumina |
TRIMMER0002_P2 |
2380 |
83-SMARTindex |
Stars ☆ |
ASV Stars ☆ |
Replicate Stars ☆ |
Percentile ☆ |
2.68 |
1.52 |
1.16 |
TRIMMER0002_P2 |
Amino Acid |
ANARCI prediction of IMGT amino acids 1-127 |
ASH*KHTDMSVPTQVLGLLLLWLTGARCDIQMTQSPASLSASVGETVTITCRASENIYSYSACYQQKQGKSPQLLVYNAKTLPEGVPSRFSGSGSGTQFSLKINSLQPEDFGSYYCQHQYGTPWTFGGGTKLEIKRA |
DIQMTQSPASLSASVGETVTITCRASENI------YSYSACYQQKQGKSPQLLVYNA-------KTLPEGVP-SRFSGSG--SGTQFSLKINSLQPEDFGSYYCQHQYG----TPWTFGGGTKLEIK |
IMGT amino acid positions:
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
22 |
23 |
24 |
25 |
26 |
27 |
28 |
29 |
30 |
31 |
32 |
33 |
34 |
35 |
36 |
37 |
38 |
39 |
40 |
41 |
42 |
43 |
44 |
45 |
46 |
47 |
48 |
49 |
50 |
51 |
52 |
53 |
54 |
55 |
56 |
57 |
58 |
59 |
60 |
61 |
62 |
63 |
64 |
65 |
66 |
67 |
68 |
69 |
70 |
71 |
72 |
73 |
74 |
75 |
76 |
77 |
78 |
79 |
80 |
81 |
82 |
83 |
84 |
85 |
86 |
87 |
88 |
89 |
90 |
91 |
92 |
93 |
94 |
95 |
96 |
97 |
98 |
99 |
100 |
101 |
102 |
103 |
104 |
105 |
106 |
107 |
108 |
109 |
110 |
111 |
112 |
113 |
114 |
115 |
116 |
117 |
118 |
119 |
120 |
121 |
122 |
123 |
124 |
125 |
126 |
127 |
D |
I |
Q |
M |
T |
Q |
S |
P |
A |
S |
L |
S |
A |
S |
V |
G |
E |
T |
V |
T |
I |
T |
C |
R |
A |
S |
E |
N |
I |
- |
- |
- |
- |
- |
- |
Y |
S |
Y |
S |
A |
C |
Y |
Q |
Q |
K |
Q |
G |
K |
S |
P |
Q |
L |
L |
V |
Y |
N |
A |
- |
- |
- |
- |
- |
- |
- |
K |
T |
L |
P |
E |
G |
V |
P |
- |
S |
R |
F |
S |
G |
S |
G |
- |
- |
S |
G |
T |
Q |
F |
S |
L |
K |
I |
N |
S |
L |
Q |
P |
E |
D |
F |
G |
S |
Y |
Y |
C |
Q |
H |
Q |
Y |
G |
- |
- |
- |
- |
T |
P |
W |
T |
F |
G |
G |
G |
T |
K |
L |
E |
I |
K |
LFR1 |
CDR-L1 |
LFR2 |
CDR-L2 |
LFR3 |
CDR-L3 |
LFR4 |
Residues |
Region |
Sequence Fragment |
Length |
Max Index Range |
1-26 |
LFR1 |
DIQMTQSPASLSASVGETVTITCRAS |
26 |
26 |
27-38 |
CDR-L1 |
ENIYSY |
6 |
12 |
39-55 |
LFR2 |
SACYQQKQGKSPQLLVY |
17 |
17 |
56-65 |
CDR-L2 |
NAK |
3 |
10 |
66-104 |
LFR3 |
TLPEGVPSRFSGSGSGTQFSLKINSLQPEDFGSYYC |
36 |
39 |
105-117 |
CDR-L3 |
QHQYGTPWT |
9 |
13 |
118-128 |
LFR4 |
FGGGTKLEIK |
10 |
10 |
|
Sequence: 2 -- Star rating: ☆3.47 -- Verified by Cloning: ✅ |
ACTGATCAGTCTCCTCAGGCTGTCTCCTCAGGTTGCCTCCTCAAAATGAAGTTGCCTGTTAGGCTGTTGGTGCTGATGTTCTGGATTCCTGCTTCCAGCAGTGATGTTTTGATGACCCAAACTCCACTCTCCCTGCCTGTCAGTCTTGGAGATCAAGCCTCCATCTCTTGCAGATCTAGTCAGAGCATTGTACATAGTAATGGAAACACCTATTTGGAATGGTACCTGCAGAAACCAGGCCAGTCTCCAAAGCTCCTGATCTACAAAGTTTCCAACCGATTTTCTGGGGTCCCAGACAGGTTCAGTGGCGGTGGATCAGGGACAGATTTCACACTCAAGATCAACAAAGTGGAGGCTGAGGATCTGGGAGTTTATTACTGCTTTCAAGGTTCACATGTTCCTCACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGGGCT |
Sequence 2 corresponding to IMGT amino acids 1-127: |
GATGTTTTGATGACCCAAACTCCACTCTCCCTGCCTGTCAGTCTTGGAGATCAAGCCTCCATCTCTTGCAGATCTAGTCAGAGCATTGTACATAGTAATGGAAACACCTATTTGGAATGGTACCTGCAGAAACCAGGCCAGTCTCCAAAGCTCCTGATCTACAAAGTTTCCAACCGATTTTCTGGGGTCCCAGACAGGTTCAGTGGCGGTGGATCAGGGACAGATTTCACACTCAAGATCAACAAAGTGGAGGCTGAGGATCTGGGAGTTTATTACTGCTTTCAAGGTTCACATGTTCCTCACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAA |
Sequencing Info
☆ Scoring Info
Amino Acid Information
Blat Sequence
Percent Support |
ASV Count |
Platform |
Plate |
Total Reads |
SMARTindex |
23.40% |
556 |
Illumina |
TRIMMER0002_P2 |
2380 |
83-SMARTindex |
Stars ☆ |
ASV Stars ☆ |
Replicate Stars ☆ |
Percentile ☆ |
3.47 |
0.47 |
3.00 |
TRIMMER0002_P2 |
Amino Acid |
ANARCI prediction of IMGT amino acids 1-127 |
TDQSPQAVSSGCLLKMKLPVRLLVLMFWIPASSSDVLMTQTPLSLPVSLGDQASISCRSSQSIVHSNGNTYLEWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGGGSGTDFTLKINKVEAEDLGVYYCFQGSHVPHTFGGGTKLEIKRA |
DVLMTQTPLSLPVSLGDQASISCRSSQSIVHS-NGNTYLEWYLQKPGQSPKLLIYKV-------SNRFSGVP-DRFSGGG--SGTDFTLKINKVEAEDLGVYYCFQGSH----VPHTFGGGTKLEIK |
IMGT amino acid positions:
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
22 |
23 |
24 |
25 |
26 |
27 |
28 |
29 |
30 |
31 |
32 |
33 |
34 |
35 |
36 |
37 |
38 |
39 |
40 |
41 |
42 |
43 |
44 |
45 |
46 |
47 |
48 |
49 |
50 |
51 |
52 |
53 |
54 |
55 |
56 |
57 |
58 |
59 |
60 |
61 |
62 |
63 |
64 |
65 |
66 |
67 |
68 |
69 |
70 |
71 |
72 |
73 |
74 |
75 |
76 |
77 |
78 |
79 |
80 |
81 |
82 |
83 |
84 |
85 |
86 |
87 |
88 |
89 |
90 |
91 |
92 |
93 |
94 |
95 |
96 |
97 |
98 |
99 |
100 |
101 |
102 |
103 |
104 |
105 |
106 |
107 |
108 |
109 |
110 |
111 |
112 |
113 |
114 |
115 |
116 |
117 |
118 |
119 |
120 |
121 |
122 |
123 |
124 |
125 |
126 |
127 |
D |
V |
L |
M |
T |
Q |
T |
P |
L |
S |
L |
P |
V |
S |
L |
G |
D |
Q |
A |
S |
I |
S |
C |
R |
S |
S |
Q |
S |
I |
V |
H |
S |
- |
N |
G |
N |
T |
Y |
L |
E |
W |
Y |
L |
Q |
K |
P |
G |
Q |
S |
P |
K |
L |
L |
I |
Y |
K |
V |
- |
- |
- |
- |
- |
- |
- |
S |
N |
R |
F |
S |
G |
V |
P |
- |
D |
R |
F |
S |
G |
G |
G |
- |
- |
S |
G |
T |
D |
F |
T |
L |
K |
I |
N |
K |
V |
E |
A |
E |
D |
L |
G |
V |
Y |
Y |
C |
F |
Q |
G |
S |
H |
- |
- |
- |
- |
V |
P |
H |
T |
F |
G |
G |
G |
T |
K |
L |
E |
I |
K |
LFR1 |
CDR-L1 |
LFR2 |
CDR-L2 |
LFR3 |
CDR-L3 |
LFR4 |
Residues |
Region |
Sequence Fragment |
Length |
Max Index Range |
1-26 |
LFR1 |
DVLMTQTPLSLPVSLGDQASISCRSS |
26 |
26 |
27-38 |
CDR-L1 |
QSIVHSNGNTY |
11 |
12 |
39-55 |
LFR2 |
LEWYLQKPGQSPKLLIY |
17 |
17 |
56-65 |
CDR-L2 |
KVS |
3 |
10 |
66-104 |
LFR3 |
NRFSGVPDRFSGGGSGTDFTLKINKVEAEDLGVYYC |
36 |
39 |
105-117 |
CDR-L3 |
FQGSHVPHT |
9 |
13 |
118-128 |
LFR4 |
FGGGTKLEIK |
10 |
10 |
|